Extraction of Heme A, Heme B and Heme O?

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Separation of Cholesteryl esters from alkanes/alkenes?

Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...

02 June 2024 5,545 0 View

EcoRV resistance E. coli?

Greetings I am working with a plasmid encoding the restriction enzyme EcoRV. Do you know of E. coli strain resistance to EcoRV restriction?

06 January 2024 8,223 4 View

"Tryptone" peptide ans casamino acids - are they phosphorylated?

Greetings all. As you probably know the "Tryptone" used as component of LB media and alike are the product of digestion of casein. Similarly Cas-Amino acids are the product of acidic hydrolysis of...

09 January 2022 2,002 1 View

Help in using MG-RAST database ?

Greetings all I am looking for a way to search for specific gene(s) expression levels in an environmental transcriptome data posted on MG-RAST. If anyone has expertise in doing such it would be...

09 May 2020 2,581 0 View

Comercial "stratum corneum substitute" lipid mixture?

Greetings We want to look at the effect of Stratum Corneum composition on bacterial attachment. Does anyone know a commercially available "stratum corneum substitute" lipid mixture? Or is willing...

17 November 2019 1,539 2 View

Origin of 220nm absorbance peak of free amino acids?

Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram

17 June 2018 5,773 3 View

Fluorophore with excitation at the UVC range and emission in the visible range?

Greetings all I am looking for a fluorophore with excitation at the UVC range (250~260nm) and emission in the UVA/visible range. Ideally one that will not bleach easily. Many thanks Yoram

05 March 2018 1,528 0 View

Energetic cost of biomass pretreatment (especialy steam explosion)?

Greetings I’m looking for numbers (rather then general statments) on the energetic cost of different biomass pretreatments before enzyme digestion for sugar release. I am especialy intrested in...

28 January 2018 5,810 5 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

19 October 2017 6,463 9 View

Microwave transperment 3D resin?

Greetings We need to create a 3D structure that will be used inside a microwave (and will contain plant biomass suspended in water). What polymer (e.g. ABS, nylon, other) would be microwave...

16 May 2017 5,216 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View