Dear Divyasree,
DMEM with a range between 1 or 4.5 g/L of glucose and 10%FBS works great for me.
But I usually work with 4g/l glucose, 580 mg/ L-glutamine [4 mM], and the FBS.
Hope it helps. Best wishes
BV-2 can be maintained easily in DMEM high glucose (4.5 g/L), 10% HI-FBS.
In the original culture conditions by the group that cloned it, the cell line was maintained in RPMI (which has 2 g/L glucose according to Invitrogen).
http://www.sciencedirect.com/science/article/pii/S0167488904002265
http://www.jleukbio.org/content/81/1/108.full
http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.114.html
Thank you so much Marc and Nai-Kei Wong.
As per my protocol I have to culture the BV2 cels in normal glycemic condition (5.5mM/L of glucose ie; 1g/L). But when I started using this concentration of Glucose the cells started dying and i could it maintain it for more than 4 days. But its growing fine in high glucose media.
To add another thought: the cells will consume the glucose available, and this will depend on the number of cells you have in x-ml of culture. Therefore, low amounts of glucose may be used up after 4 days.
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