This micrograph was prepared by SEM from human dermal fibroblast which was cultured on special scaffold.
It is impossible to tell whether two specific particles on your pictures are exosomes. And you have a lot of them, and they are of different sizes. I would rather suspect that something went wrong with specimen preparation or that there are a lot of apoptotic cells in your culture.
Dear Amrollah, as a morphological view, your particles can be easily exosomes, since they fall into 50-200nm. However, as your cell is almost covered with them, it is a bit weird why are there so many. More details about the sample preparation or treatment would help.
Best,
Attila
It is impossible to tell whether two specific particles on your pictures are exosomes. And you have a lot of them, and they are of different sizes. I would rather suspect that something went wrong with specimen preparation or that there are a lot of apoptotic cells in your culture.
If your scale is correct at 10 um, virtually none of the visible particles are exosomes. Atilla has the correct size range for exosomes but by my estimation the arrows seem to point to objects around 500nm upwards. There are some smaller particles that may begin to fit into the top of the exosomal range however even they are few and far between. You really need a higher power image to see exosomal populations.
In fact, there are also likely to be non-exosome particles that also sit within the 30-200nm range so to truly identify exosomes from EM alone you should use immuno-gold staining or similar, with antibodies directed towards known exosome markers; alix and CD9 for example. I've not seen SEM immuno-gold imaging but I understand that there are protocols, TEM immuno-gold imaging is more common for isolated exosomes.
The magnification is too low and it is impossible to say "these particles are exosomes". It can be everything.
hi. why dont you do some molecular analysis, as everyone says it is hard to say from just a image, exosome have markers on their surface and you can use them to do flow cytometry for checking
Dear Vahid (having had troubles the whole day [Mon,2017-02-13] to answer due to a blocked ADD YOIR ANSWER-function....but this could have had its origin in a java-script problem on my PC) ....,
according to the included measurement bar the underlying longitudinal textures in your enclosed picture kia(2).bmp are approximately 1-1.2 µm long.
I admit that I never extracted exosomes from (cancer) cells, especially by using an "ExoSpin kit" so I greatly should appreciate your determining / naming those "bacteroid" structures (for 'enhancing' and completing my knowledge).... since I am sure that those bacterioid structures cannot be or 'are' 'cancer cells' (cf. e. g. SEM -images of MDA-MB-231 cells in https://www.researchgate.net/publication/23670506_Cytoskeletal_role_in_differential_adhesion_patterns_of_normal_fibroblasts_and_breast_cancer_cells_inside_silicon_microenvironments/figures?lo=1 ) I only can guess: could these structures perhaps be mitochondria? Thank you for clarification
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