Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Do I need to correct Sentinel images (_MSIL2A products)?

Hi guys. I have some questions for you. I've seeing a lot of people using sentinel 2A images without atmospheric correction because they say _MSIL2A products are already corrected. However,...

07 December 2020 2,118 2 View

Can anyone help me with a bootstrap resampling problem in TNT?

Greetings, Can anyone help me with a bootstrap resampling problem in TNT? I am currently running an analysis with TNT in a data matrix composed by morphological characters (105 discrete and 25...

23 October 2019 6,645 3 View

Can anyone help me histology TRAP?

Can anyone help me histology TRAP? I'm doing the manufacturer's protocol (SIGMA), and the coloring turns purple. Does anyone know what could be wrong?

09 July 2019 8,894 3 View

Can anyone help me with microplates reading?

I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...

06 May 2019 3,093 11 View

How to merge two different flowcell of fastq?

Hello, I need to merge two fastq files, which belong to two flowcell, one file has 71 ind and the other 96, so in total they are 167 ind. How can I merge them? I'm analyzing the cyverse data with...

25 November 2018 4,672 3 View

Methods for 3D culture of Hs578T cells?

Hey! I'm currently performing my dissertation using Hs578T cells and I would like to try to perform the mammosphere assay. Has anyone ever worked with this cell line and did this assay? If so,...

18 September 2018 4,619 3 View

What is tRNA level in strain Rosetta (DE3) since it supplies tRNAs for rare codons?

tRNA level in E. coli BL21 strain is ~15% total. I wonder what is tRNA fold increase in strain Rosetta (DE3) since it carries pRARE plasmid.

14 August 2018 5,946 2 View

How to preserve best PBMC samples for performing FACS staining later ON?

08 January 2018 6,965 7 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Can people point me to interpretative phenomenological analysis (IPA) studies where there are only two participants?

I am in search of studies with a small n to support recent work completed in-depth with two participants regarding international students in Canada. I am not sure if framing things as two case...

23 January 2021 8,564 7 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

How to obtain a wet deposited film by spin coating method?

I tried to deposit some polystyrene thin films by the use of spin coating method. I should mention that the solvent which I used was toluene. The films which I obtained were totally dry. While I...

14 December 2020 4,655 5 View

Does anyone knows the solubility or miscibility of xylene in tetrachloroethylene?

I need to learn if xylene and tetrachloroethylene are miscible or not. It is also will be usefull to learn a solvent that is not miscible with tetrachloroethylene. Thanks,

09 December 2020 3,383 2 View

Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do I make nano-sized particles of Polyethylene?

I am attempting to make particles in the range of 50-500 nm from Low density polyethylene. My current protocol is to dissolve 0.1 g of LDPE in xylene at 100 C, then drip the resultant solution...

08 November 2020 7,764 1 View

Which is preferable for whole rat brain clearing: tert-butanol or xylene?

I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.

05 October 2020 1,306 1 View

How is solubility of CO2 or air in organic solvents such as xylene or cyclohexane?

For some reason, I need to inject a dispersion of an organic solvent and organoclay into a block of soil sample. the organic solvent probably is xylene.

29 September 2020 6,348 4 View

Is Cucujus clavipes (Coleoptera: Cucujidae) legaly protected or included in the Red Data List/Book in any state of the USA or Canada?

Most (especially European and Asiatic) Cucujus species (Coleoptera: Cucujidae) are known as rare or threatened or even endangered. Is the same situation in case of the American Cucujus clavipes...

15 September 2020 9,190 4 View