I want to count the number of germinated fungi spores, but I'm having trouble with the difference between the alive and the dead spore
Have you tried a live/dead stain? Not sure how well it works for fungal spores.
Article A rapid method for assessing the viability of fungal spores1
I want to know about herbivore-induced plant volatiles from a plant attacked by pests. Is there any chance for me to know what kinds of compounds the plant produces and emits in real time? Thank...
27 June 2024 5,162 5 View
Dear Research gate, I just found that our unpublished manuscript is in the pre print. I am wondering why that title can be obtained by ResearchGate as well as our manuscript. Since we are still...
18 March 2024 8,435 2 View
I stored blood sample at -88 five months ago, I would like to ask if there is possibility to thaw them again for isolation of exosomes?
06 November 2023 9,921 2 View
Keadilan lingkungan merupakan suatu hal yang sangat penting bagi masyarakat. Lingkungan yang sehat dan lestari akan memberikan manfaat bagi kehidupan manusia . Namun , seringkali kepentingan...
01 June 2023 8,643 0 View
Greeting I have to three lung cancer cell lines with low metastasis and I need some highly metasttic lung cance cell lines. Gow I can transfer these low metastatic cancer cells to highly...
19 October 2022 1,303 5 View
What is the best technique to treat regular lung cell lines with exosomes?
15 June 2022 9,408 0 View
Glycerol monostearate (GMS) is insoluble in water and soluble in hot ethanol. Therefore, in most formulations of solid lipid nanoparticles (SLNs) using GMS via ultrasonication and high pressure...
22 May 2022 8,702 1 View
Hello everyone, There are two parts to this question: 1. How to calculate misfit between two phases in different lattice structures (e.g. A phase in hcp vs B phase in bcc) from High-Angle...
24 May 2021 3,111 2 View
Dear everyone, I am relatively new to cell culture, and I need some help. At the moment, I am culturing RAW 264.7 cell line. I have some question regarding this. I am not quite sure about the...
17 May 2021 3,457 14 View
Insulin is the only known hormone that can directly promote anabolism and reduce blood glucose levels. I am curious as to whether there exist other hormones with the same function as insulin? If...
20 April 2021 2,399 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...
15 June 2024 6,693 0 View