Hello Dian Jamel

The metabolic differences among cancer cells confer differences in their metastatic potential. Metastatic cancer cells depend on monocarboxylate transporter 1 (MCT1) to deal with oxidative stress. MCT1 plays a major role in circulating lactate, which is a prominent energy source for metastasizing cells. As such, highly metastatic cells have increased levels of MCT1, whereas the inhibition of MCT1 decreases lactate uptake by metastatic cells and, thus, reduces their metastatic capability.

Also, changes in ATP/ADP and ATP/AMP ratios promote metastatic behavior.

So, having high levels of MCT1 on the surface of cells may probably help making the cells highly metastatic. Give it a thought.

Best.

Consider the cellular transformation by Oncogenic RAS and MMPs. Try Stepwise transfections.

Dear Malcolm Nobre

I do not think that just by making a cell express METS the metastatic rate will increase. Metastasis is far more complex than that. On the other hand as Naseer says, transferring Ras can increase the number of metastasis. The simplest and cheapest way that can tested is to cultivate cells at a low oxygen level. Between 1 and1.5%. For a long time. Other option is concomitant culture in low oxygen environment with a pH under 6.5. Hypoxia plus acidity increase the metastatic phenotype.

But how will the cells grow under these conditions? Will it not die?

To generate highly metastatic lung cancer cell lines from low metastatic ones in vitro, you can employ a process called in vitro selection or serial passaging. Here's a general outline of the approach:

It's important to note that the metastatic potential of cell lines generated through in vitro selection may not fully recapitulate the complex processes observed in vivo. Therefore, it is crucial to validate the findings using relevant in vivo models and compare the results with patient-derived samples or established highly metastatic cell lines.