What is the best technique to treat regular lung cell lines with exosomes?
I want to know about herbivore-induced plant volatiles from a plant attacked by pests. Is there any chance for me to know what kinds of compounds the plant produces and emits in real time? Thank...
27 June 2024 5,162 5 View
Is it worth using a paired t-test on pre and post survey data with 12 or less responses? Or should one rely more on the mean and standard deviation of the two data sets to evaluate the survey...
27 April 2024 8,703 8 View
Dear Research gate, I just found that our unpublished manuscript is in the pre print. I am wondering why that title can be obtained by ResearchGate as well as our manuscript. Since we are still...
18 March 2024 8,435 2 View
"From science to law, from medicine to military questions, artificial intelligence is shaking up all our fields of expertise. All?? No?! In philosophy, AI is useless." The Artificial Mind, by...
22 February 2024 9,958 98 View
Impact factor of IJERR
05 January 2024 1,403 2 View
Please, how is the impact factor of european chemical bulletin? With wishes
03 January 2024 1,641 1 View
I stored blood sample at -88 five months ago, I would like to ask if there is possibility to thaw them again for isolation of exosomes?
06 November 2023 9,921 2 View
Keadilan lingkungan merupakan suatu hal yang sangat penting bagi masyarakat. Lingkungan yang sehat dan lestari akan memberikan manfaat bagi kehidupan manusia . Namun , seringkali kepentingan...
01 June 2023 8,643 0 View
I want to count the number of germinated fungi spores, but I'm having trouble with the difference between the alive and the dead spore
31 March 2023 2,960 1 View
to optimize the space as much as possible and for ergonomic reasons, it is necessary to design a cold room that is higher than it is wide.
26 February 2023 5,041 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View