I did Y2H experiment. negative control could not grow on QDO+X-alpha-Gal while positive control and my BD-gene+AD-gene could grow on QDO+X-alpha-Gal but did not turn blue.
I'm currently working on complementary medicine for treating PCOS related infertility and reducing Insulin Resistance. And my Professor wants me to publish a literature review on Complementary...
06 January 2020 2,612 3 View
Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...
11 February 2021 3,292 3 View
Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...
07 February 2021 8,258 3 View
I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...
01 February 2021 9,936 2 View
Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...
19 January 2021 4,566 2 View
I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...
04 January 2021 9,669 2 View
Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...
28 December 2020 4,309 11 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.
22 December 2020 1,432 2 View
I performed a digestion that ended up at midnight, but forgot to inactivate the enzymes (HaeIII and RSAI) before freezing the products. I plan to run a gel to differentiate yeast isolates. Can I...
09 December 2020 3,551 3 View
Hello, I am new to yeast research. I am trying to rescue plasmid from yeast and have tried several protocols developed by researchers. But the results are not promising. I ended up buying this kit...
06 December 2020 1,251 2 View