I did yeast two hybrid transformation a few days ago and no yeast colonies grow in the DDO+ 225 AbA plates after 3-5 days. OD600, carrier DNA, competent cell, as well as the amount of AD and BD plasmid were correct according to TAKARA manual. Can anyone know the reason why no colonies could grow in the plate?
Amy Klocko
Try giving it a few more days. Sometimes you'll need 7-10 to see some growth. Also, try making single plasmid selection plates to check that both your bait and prey plasmids are giving good transformation and growth on their own.
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Faujiah Nurhasanah Ritonga
thank you for your answer Amy. I also tried to make single plasmid selection but still no yeast colony grow. I will wait 7-10 days more.
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