Hi everyone, I am working on developing a HPLC method for quantifying amino acids in a DP that has 8% sucrose.Any leads on how to remove the sucrose?

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Charles H Hocart

Use ion exchange SPE to retain the AA and allow the neutral sucrose to pass through unretained with the sample wash - lots of application notes out there from chromatography companies

Gurnoor Judge

Thank you ! this definitely helps!

Mehul Sharma

which type of detector are you using it meters, and where your negative peak arrived it matters too.