Hi dear colloquies , do you know how to isolate T or B lymphocytes with particular CD marker ?

More Luma Hassan Alwan Alobaidy's questions See All

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Where can I find clear SOP for anaerobic bacteria regards below questions?

* What is the survival time of anaerobic bacteria especially that isolated from maxillofacial an hip replacement surgeries in an aerobic environment? what is the best transport media to use ( is...

23 November 2022 5,979 2 View

Hi dear colloquies, I am working on GRHPR enzyme , I already measure the concentration by ELISA , did anyone measure the enzyme in serum by ELISA?

Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase its defeciancy lead to accumulation of oxalate.

07 October 2022 8,908 0 View

What are the factors affecting on the a cyclization closure of schiff base compound?

I want to react imine bond (schiff base compound) with thioglycolic acid and there is ortho substituent (-C2H5) in amine sction . Could this reaction happen easily? Could ortho- C2H5 substituent...

11 June 2022 9,613 5 View

Hi dear colloquies, Please, how to identify the LD50 for different plant extracts in tissue culture ?

Hi, I want to study the LD50 and cytotoxicity of an alcoholic , water extracts and the oil of plant in tissue culture and which lab animal is the suitable?

04 September 2021 527 2 View

Is there any non-ionic surfactant that insoluble in water?

I am looking for an experimental work that deal with non-ionic insoluble surfactant in O/W emulsion. All the non-ionic surfactants are soluble in water. I am wondering if there any non-ionic...

23 December 2020 929 1 View

Who are working in fault diagnosis for a robot systems?

who are working in fault diagnosis for a robot systems? which methods did you use which environment is the best?

01 November 2019 6,396 3 View

How can I calculate a zero point charge of photocatalysts in simple method????

I need to simple method to study a zero point charge of photocatalysts...

09 December 2018 9,945 7 View

Why the score value in research gate decreases sometimes during weekly updated?

why the score value in research gate depresses with weekly updated, sometime?

20 May 2018 2,220 92 View

Why the quantum yield decrease after addition of H2O2 to dye solution?

The quantum yield decreases with addition of oxidant agents like H2O2 to solution of some dyes.

29 March 2018 8,134 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View