Dear Luma

First of all it is very important to know that on which cells you are going to study the cytotoxicity activity then only then only it will be decided that which animal and animal model you should use.

Before going to study LD50 of your sample I will suggest first go for maximum tolerated dose on animal models and then it is important which assay you will be using for cytotxicity activity.

Hi dear Dr Luma, Thanks for your excellent question, I attached some references and also refering the same question and page here in Research gate, Hope they could be useful for you, otherwise please tell me to present another

1- https://www.researchgate.net/post/What-LD50-in-a-cellular-toxicity-assay-of-a-plant-extract-should-be-considered-as-low-toxicity

2- Article Assessment of cytotoxicity exerted by leaf extracts from pla...