Hi, can anyone tell me that how to confirm the cell wall or membrane inhibition in aspergillus other than SEM or TEM imaging?. Thanks in advance.?

More Dhanaraju Mandalapu's questions See All

How to remove excess fluorescent probe from LUVs?

Hi all, I would like to know how to remove the excess fluorescent probe from the LUVs prepared after hydration? And how to do it, any procedure to recommend? Thanks.

10 November 2018 6,311 0 View

How to check the bacterial membrane potential?

How to check the bacterial membrane potential? I am looking for some dye based assays where i can observe fluorescence fluctuation, and with my initial trails with safranin O didn't succeeded. I...

04 May 2018 4,238 4 View

How to prepare the aspergillus fungal sample for SEM analysis after treating with synthetic compounds. How to transfer the compounds treated fungi ?

How to transfer the compounds treated aspergillus to sample preparation, where the activity assay was Carried out on agar plates?

03 April 2018 9,464 2 View

How to check Bacterial cellwall inhibition?

How to check the bacterial cellwall inhibition mechanism of an active peptide compound. I know two methods. 1) Fluorescence labelling 2) SEM-imaging If the compound hasn't any reactive functional...

02 March 2018 7,641 0 View

Purification of bacterial natural peptides using RPHPLC-flash chromatography

I would like to know is it possible to isolate polypeptide and large molecular weight natural product peptides from bacterial and fugal culture broths using RPHPLC-flash chromatography? most of...

01 January 1970 6,563 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Denis Korneev

I don't have any experience with aspergillus, but the main principles are the same for all living cells:

1. Differential staining for conventional light microscopy. It is a well-known technique and there are a lot of protocols and commercial chemicals.

2. Biophysical methods. Cell wall or cell membrane conductivity is strictly depend on their conditions and can be studied by dielectrophoresis. For example:

https://www.ncbi.nlm.nih.gov/pubmed/8318523

http://jb.asm.org/content/162/2/790.full.pdf

Good luck!

Dhanaraju Mandalapu

Thanks Denis