I would like to know is it possible to isolate polypeptide and large molecular weight natural product peptides from bacterial and fugal culture broths using RPHPLC-flash chromatography? most of the times using for non-peptides. if its possible, are there any suggestions for good instrument. Thanks in advance.
http://www.flash-purification.com/peptide-purification-with-flash-column-chromatography-a-beginners-experience/
HI,
yes, it is possible.
I usually use MeoH/ water gradients. And C8 cartridges instead of C18 if possible, than it works pretty fine.
Best regards, Eva.
@eva do you recommend any references?
Thanks for the answer
Raju.
I've also have done it. I've used the technique to increase the loading for prep HPLC. The flash worked well enough that prep HPLC just needed "polishing".
Please see: https://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/Pre-Purify%20Peptides%20with%20Flash%20Chromatography.pdf#search=peptide
- Badges
- Science topic
- Similar topics
- Instrumentation Engineering
- Instruments