Hi all,
I would like to know how to remove the excess fluorescent probe from the LUVs prepared after hydration? And how to do it, any procedure to recommend? Thanks.
How to check the bacterial membrane potential? I am looking for some dye based assays where i can observe fluorescence fluctuation, and with my initial trails with safranin O didn't succeeded. I...
04 May 2018 4,238 4 View
Need protocol or suggestions
03 April 2018 4,542 2 View
How to transfer the compounds treated aspergillus to sample preparation, where the activity assay was Carried out on agar plates?
03 April 2018 9,464 2 View
How to check the bacterial cellwall inhibition mechanism of an active peptide compound. I know two methods. 1) Fluorescence labelling 2) SEM-imaging If the compound hasn't any reactive functional...
02 March 2018 7,641 0 View
I would like to know is it possible to isolate polypeptide and large molecular weight natural product peptides from bacterial and fugal culture broths using RPHPLC-flash chromatography? most of...
01 January 1970 6,563 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View