How to prepare the aspergillus fungal sample for SEM analysis after treating with synthetic compounds. How to transfer the compounds treated fungi ?

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How to remove excess fluorescent probe from LUVs?

Hi all, I would like to know how to remove the excess fluorescent probe from the LUVs prepared after hydration? And how to do it, any procedure to recommend? Thanks.

10 November 2018 6,202 0 View

How to check the bacterial membrane potential?

How to check the bacterial membrane potential? I am looking for some dye based assays where i can observe fluorescence fluctuation, and with my initial trails with safranin O didn't succeeded. I...

04 May 2018 4,150 4 View

Hi, can anyone tell me that how to confirm the cell wall or membrane inhibition in aspergillus other than SEM or TEM imaging?. Thanks in advance.?

Need protocol or suggestions

03 April 2018 4,477 2 View

How to check Bacterial cellwall inhibition?

How to check the bacterial cellwall inhibition mechanism of an active peptide compound. I know two methods. 1) Fluorescence labelling 2) SEM-imaging If the compound hasn't any reactive functional...

02 March 2018 7,545 0 View

Purification of bacterial natural peptides using RPHPLC-flash chromatography

I would like to know is it possible to isolate polypeptide and large molecular weight natural product peptides from bacterial and fugal culture broths using RPHPLC-flash chromatography? most of...

01 January 1970 6,482 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View