Dhanaraju Mandalapu

6 Questions 8 Answers 0 Followers

Questions related from Dhanaraju Mandalapu

How to remove excess fluorescent probe from LUVs?

Hi all, I would like to know how to remove the excess fluorescent probe from the LUVs prepared after hydration? And how to do it, any procedure to recommend? Thanks.

11 November 2018 6,270 0 View

How to check the bacterial membrane potential?

How to check the bacterial membrane potential? I am looking for some dye based assays where i can observe fluorescence fluctuation, and with my initial trails with safranin O didn't succeeded. I...

05 May 2018 4,203 4 View

Hi, can anyone tell me that how to confirm the cell wall or membrane inhibition in aspergillus other than SEM or TEM imaging?. Thanks in advance.?

Need protocol or suggestions

04 April 2018 4,516 2 View

How to prepare the aspergillus fungal sample for SEM analysis after treating with synthetic compounds. How to transfer the compounds treated fungi ?

How to transfer the compounds treated aspergillus to sample preparation, where the activity assay was Carried out on agar plates?

04 April 2018 9,435 2 View

How to check Bacterial cellwall inhibition?

How to check the bacterial cellwall inhibition mechanism of an active peptide compound. I know two methods. 1) Fluorescence labelling 2) SEM-imaging If the compound hasn't any reactive functional...

03 March 2018 7,602 0 View

Purification of bacterial natural peptides using RPHPLC-flash chromatography

I would like to know is it possible to isolate polypeptide and large molecular weight natural product peptides from bacterial and fugal culture broths using RPHPLC-flash chromatography? most of...

01 January 1970 6,531 4 View