Dear Yahya Alsalhi
Aqueous alcohol (80%) is most suitable for phenolic extraction of any fresh / fleshy fruit. For this
1. Transfer healthy fruit sample in deep freezer for 7-10 hours.
2. Chop the sample by knife.
3. Immediately after chopping, allow the fruit sample to freeze dry completely using a lyophilizer instrument.
4. Crush to make powder from lyophilized and dry fruit sample.
5. Mix powdered sample in aqueous ethanol / methanol vigorously.
6. After suitable incubation in shaking incubator at optimum temperature, filter the mixture through Whatman filter paper.
7. Prepare a composite filtrate by repeating former two steps three or four times.
8. Condense the composite filtrate using Rotary evaporator and then use as working extract for further tests/analysis or store at low temperature.
http://arrow.dit.ie/cgi/viewcontent.cgi?article=1098&context=schfsehart
Dear Yahya Alsalhi
Aqueous alcohol (80%) is most suitable for phenolic extraction of any fresh / fleshy fruit. For this
1. Transfer healthy fruit sample in deep freezer for 7-10 hours.
2. Chop the sample by knife.
3. Immediately after chopping, allow the fruit sample to freeze dry completely using a lyophilizer instrument.
4. Crush to make powder from lyophilized and dry fruit sample.
5. Mix powdered sample in aqueous ethanol / methanol vigorously.
6. After suitable incubation in shaking incubator at optimum temperature, filter the mixture through Whatman filter paper.
7. Prepare a composite filtrate by repeating former two steps three or four times.
8. Condense the composite filtrate using Rotary evaporator and then use as working extract for further tests/analysis or store at low temperature.
http://arrow.dit.ie/cgi/viewcontent.cgi?article=1098&context=schfsehart
Most of polyphenols requires tailored procedures for the follow-up of purification by NMR and HPLC. http://www.sciencedirect.com/science/article/pii/S2095177912001426 dry fruits can be obtained by freeze dry using CO2 dryice and alcool solutions or liquid nitrogen on residual from a juice press.
Please make also sure that you lower the pH of your extracts < 3. Above a pH of 3 phenols tend to oxidise much more rapidly.
It would be possible to dry your extract by using a suitable freeze dried
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- Analytical Chemistry
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