while get small quantity of jam polyphenol crude extracts after concentrate via rotary evaporator process. Is it safe to preserve that crude in eppendorf tube (-20) till next test? How to insure that there is no interact between plastic and crude?
I read an article related to dpph assay via microplate reader for a researcher ( Jose Maria Prieto ) but unfortunately i did not get the references for that paper. so kindly anybody have that...
31 December 2017 6,055 0 View
Dear all I have three plant samples .each one had extracted with different solvents like ethanol,acetone and methanol. If I want to measure and compare their antioxidant activities of these...
08 September 2017 4,355 4 View
Hello everybodyI did total polyphenol content for my plant extract . I used Gallic acid as standard and the (OD)s were Gallic acid standard (mg/ml) blank. = 0.0000Conc. 0.25 =0.00350Conc. 0.5=...
08 September 2017 6,173 0 View
Dear all. many research papers regarding quantitative of total phenol content in plant samples vary in use wavelengths (uv vis)spectrophotometer (750nm) .some papers use ( 650nm). Unfortunately...
07 August 2017 7,471 4 View
Dear all I have crude extract of several plant samples.the previous extract done by different solvent with different ratio(liquid/liquid).now I want to detect the content of...
07 August 2017 6,303 6 View
I have different plant extracts for polyphynol .I want to reduce volumes and concentrate them by using oven dryer. I try 40c for 24 hours by little effect. Can I increase temperature for example...
05 June 2017 7,737 0 View
Stability of phenol in juicy fruit during the droughtness
01 January 1970 953 6 View
Dear all Is there any procedure to estimation and determination of amygdalin in plant extract by spectrophotometer rather than HPLC. Thank you
01 January 1970 7,248 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View