It is considered that the following protocol is ; DMEM (Gibco) + 10 % FBS )
Well, generally speaking the first growth of cell line following their awakened from cryopreservation stage is usually slow but after that, their growth will be faster if we provide their optimal growth conditions and if we alter the concentration of FBS
What passage number do you use for culturing the cells, as of now. Generally passage number over 70 is not considered for experimental setup as it changes its morphology and function. Thanks
Try a different batch of FBS. If one batch works well, call the company to reserve that batch for future. Good labs practice this simple procedure. For you, try other cell line too with the same batch of FBS if other cell line grows slow too, than you may consider different batch. As said before first cycle after thawing cells are slightly slower, after that they pick up the speed.
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