I have left over cDNA from a microarray experiment, can I use that cDNA for qPCR or should I make new cDNA from RNA?
Adeola
As long as your cDNA was prepared with qPCR compatible reagents, such as most standard reverse transcription kits, I think your cDNA should be fine. cDNA is quite stable. The only thing I would worry about is if it is conjugated/modified in any non-typical ways (such as dye labeling).
If it's the cDNA leftover directly after reverse transcription, then you can use it. For confirmation of microarray results this is the best option especially since RNA is sensitive to degradation.
Which kit did you use for making cDNA? Check the data sheet and if the kit has 2 different buffers, then you should check if that buffer is compatible for miR, precursor of miR and for mRNA.Qiagen-kit has 2 different buffers and they are used for different purpose
Hi Adeola, I have the same question to ask. Have you tried to use the cDNA from microarray for QPCR?
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