In the case of female non-avian reptiles that lay eggs and guard their nests, is there information on what hormones determine their behavior? And in the case of birds?
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,199 2 View
Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...
03 July 2024 6,176 3 View
I made a series of jump tests in subjects, everything was recorded with Vicon. I have data from markers and force plate. We recorded the tests before and after an intervention. My question is, I...
23 June 2024 9,380 4 View
Do you think the use of virtual reality with dyslexia students is good?
23 June 2024 8,437 2 View
I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.
22 June 2024 4,858 8 View
I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...
21 June 2024 3,305 6 View
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19 June 2024 2,123 3 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,906 4 View
I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...
16 June 2024 1,115 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,759 5 View
We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments
15 July 2024 9,314 2 View
Dear Researcher, I have used nested PCR to increase the specificity of Tetra Arms. Previously, I obtained results for another SNP using the same reaction conditions, and separate reactions...
14 July 2024 429 4 View
Dear colleagues I would like to know which Astigmata species I found in left cormorant nests. Could somebody help me with the identification?
31 May 2024 3,887 1 View
Additionally, could you suggest any related articles or resources on this topic?
07 May 2024 479 0 View
I am running a Nested PCR on blood DNA. Although the primers have worked successfully in previous publications, I am not getting a clear bands for sequencing. The first PCR reaction mix...
25 April 2024 4,661 3 View
For my thesis on the same HPV L1 region sequencing for detecting genotypes , I have used My09/11 & GP5+/6+ primers for amplification on Nested PCR, but I couldn't find sequences other than 16,...
19 April 2024 1,747 1 View
Can the acidity of the silicon hydroxyl group of zeolite, whether it is the terminal hydroxyl group or the silanol nest, catalyze the ethanol dehydrattion?
11 April 2024 1,235 0 View
I am looking for help to optimize a nested PCR from Long Range (LR) PCR (DNA as template). The LR PCR product looks spesific on agarose gel. We have tried various dilutions of this product as...
01 April 2024 6,834 5 View