Hello!
I'm gonna start to work with HepG2 cells and I would to know what are best culture conditions for these cells. I have been reviewed some articles and people use many types of medium and additives.
In our lab, we culture HEPG2 cells in Dulbecco’s minimal essential medium (DMEM) supplemented with 5% fetal bovine serum, penicillin (100 units/ml), streptomycin (100 μg/ml), 2mM Glutamine and 0.3% glucose. Cells are grown in a 37°C humidified incubator with 5% CO2. Hope this helps!
Hi, I agree with Srinivas but recommend starting culture with 10% FCS and then reducing to 5% after 3-5 passage.
Ok, I have a question... Should I used 5% FBS or I can use at 10%? Why is important keep the cells with FBS 5%?
Thanks
If your HepG2 cells are new (I mean, if they not being splitted before) you should use a media which doesn't contain antibiotics untill 20th passage number. Otherwise, they'll grow very slowly. New cells are very sensitive. They may even don't like the brand of the flask you're using. Moreover, after using trypsin if your cell is still not deattached, don't do cell scraping immediately(it should be a last choice). Just add 1-2 ml more trypsin and put it into incubator for about 4-5 min. (I experienced that 3 min is not sufficient)
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