Help on that bisulfite treatment protocol without using kit or restriction enzyme?

Fadwa Aljoud @Fadwa-Aljoud

03 April 2016 1 10K Report

Can anybody direct me to the best possible bisulfite treatment protocol without using kit or restriction enzyme?

Thanks

Karthik Raman

Take a look at the attached protocol for bisulfite treatment of genomic DNA. It's short, but should get the job done.

http://www.scigine.com/method.php?ID=39662

Badges
Science topic

More Fadwa Aljoud's questions See All

I have two profiles in research gate with the same name Fadwa Abd El Reheem. How can I join both together to one profile?

One with my previous position as Lecturer of Clinical Pathology in Fayoum University and the other with my current position as Associate Professor of Clinical Pathology in Fayoum University.

13 February 2024 3,713 1 View

How to analyze Total fats from grains?

I have a method using AOAC method to test dried fruits, vegetables and herbs for Total fats but it doesn't work for grains, such as oats, quinoa, breakfast cereal, etc. I get about half less than...

25 April 2022 287 16 View

Which is the best nano-encapsulation technique to cover the bitter taste of the bio-active compounds?

I tried to cover the bitterness of the bioactive compounds of a medicinal plant by encapsulating them using the nanophytosome technique putting them in a sweet food product, but it did not work.....

30 December 2021 5,590 10 View

Which temperature is suitable for the micro-metering valve (separation valve) to extract terpenoids using supercritical CO2 extractor?

I extracted a plant rich in terpenoids with Supercritical CO2 (without any co-solvent) in the condition: ( vessel temperature=50 C, seperation temperature- 120 C, and pressure= 200 bar). I did not...

22 July 2021 2,599 2 View

How can I analyze sugars in freeze-dried food using GC-FID?

I need to analyze sugars in freeze-dried food using GC-FID. I don't see any new techniques for this. I see this mainly done by HPLC-ELSD and RID. I've seen old technique whereby pyridine is...

09 June 2021 1,828 5 View

Can we apply CRISPR genome editing on cell line?

We are planing to do CRISPR but we found it is a little hard to do it in hiPSC at a moment. Can we apply it in cell line or not?

10 July 2019 1,950 2 View

What do you really think about silver nanoparticles? Are they safe to use them in food and food packaging? Or toxic to human?

I read about silver nanoparticles that they make the skin blue. But is that scientifically proven? What can cause that? And does that mean that they are toxic?

24 April 2018 2,611 3 View

Does anyone know how to use OPA as derivatizer for amino acidsif this can't be done on-line on HPLC ?

I have to develop an amino acid method for only one amino acid and I need to derivatize but the HPLC does not have an on-line way to do it. How can I derivatize manually with OPA?

12 March 2018 6,104 4 View

How can I use ( Alibaba )to buy something from china?

Is Alibaba market real? are the prices including shipping? how to make sure that they will ship the goods and they will be in a good quality and manner and will not be late?

16 February 2018 6,908 5 View

Does anyone have papers on extraction of FAMES, omega 3 and omega 6 from seeds and herbs using a GC/MS or FID?

Does anyone have papers on extraction of FAMES, omega 3 and omega 6 from seeds and herbs? I have to do this with a GC/MS or FID.

12 October 2017 7,252 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View