Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Can the intensity of this broad band in XRD mask other crystaline phases present?

I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....

19 February 2021 8,414 4 View

Heat exchange due to humidity difference ?

Hello everyone. How can I simulate evaporative cooling between air and water (isothermal) placed in direct contact? Is it possible to choose or set the humidity difference as the heat exchange...

11 November 2020 5,225 3 View

How can I simulate evaporative cooling between substances in contact using Ansys Fluent?

Hello everyone I need to simulate a counter-current flow between air and water droplets (in direct contact) which by evaporative cooling give rise to a cooling of the wall on which the droplets...

07 November 2020 5,458 2 View

TPB: Can you use indirect questions for direct measurment?

Hi, I am doing a research with Theory of Planned Behavior. The behavior is attending a course/training on the subject of orientation, navigation and topography in the next year. I completed...

18 August 2020 4,502 1 View

Is it possible to use a muffle furnace without N2 atmosphere to make Biochars?

Greetings Everyone. I am reading several articles about biochars and notice several use N2 atmosphere inside the muffle furnace. can you produce biochars without this N2 atmosphere or other inert...

17 August 2020 8,992 6 View

How to convert GeoTiff to a format compatible with FRAGSTATS?

Dear all, I need to process several classified GIS maps in FRAGSTATS. All files are in GeoTiff format. I do not have ArcGIS. I also assume that when you export files to ASCII format in QGIS often...

01 July 2020 2,433 5 View

How to prepare (good) conductive nanocontacts?

Dear all, I need to prepare electrical contacts in order to connect a gold pad with a semiconductor (few microm size) . i cannot perform lithographic process. Hence, how can I obtain good...

09 June 2020 9,756 8 View

Are COVID-19 clinical datasets available?

Do you know if (anonymised) clinical high-frequency datasets of COVID-19 patients, such as pulmonary function, blood oxygen level, blood pressure, cardiac interbeat interval etc., are available...

04 May 2020 2,195 5 View

What tunes the selectivity of ionic exchange towards an ion? can we predict it? Is it pH dependent?

Cheers Everyone. I was seeing some uses of ionic exchange to recover NH4 by ionic exchange with other cations in a specific matrix. And it brought me to question what drives an ionic exchange to...

17 February 2020 6,596 3 View

No title

02 March 2021 3,060 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

No title

22 February 2021 9,932 11 View