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Hello everyone, I typically obtain good results using the commercial silica column-based DNA extraction kit from QIAGEN (dried mushroom specimens and pure cultures). However, I’ve recently...
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Hello, I'm interested in optimizing the cost-efficiency of the purification of PCR products from macro fungi for Sanger sequencing using Exo+Sap mixture. My Exo+Sap product protocol suggests...
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The output file for the selected evolutionary models by ModelFinder is extremely confusing. This is what I got: PartitionModelPlen: 58s+tefcdn1_1+tefcdn1_2+tefcdn3_3+rpb1cdn1_1!ModelDNA!rates:...
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Hello, I'm a master student working with fungi. And one of my studies includes the evaluation of the mycelium growth efficiency and biomass production of mushroom strains on different culture...
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Considering the discrepancy of labels and compositions of commong medias, I'm looking for an informative and reliable reference (book or article) for the preparation of different culture media,...
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So, I've been doing DNA extractions and PCR of mushroom collections and cultures, and I'm having mixed results. Routinely, we've been diluting all our DNA extractions if it's above 50 ng/ul, but...
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Hello, guys. I'm a master student working with the domestication of wild strains of edible mushrooms from Brazil and during the process of isolation and maintenance of the strain cultures,...
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