Help needed. E.coli Nissle strain wanted.

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How can I make the CST simulation run faster?

I am trying to simulate a metasurface of 24x24 elements, but even with a GPU of 48GB it takes very long to simulate. I want to obtain the field along the defined curve.

10 June 2024 3,577 7 View

Why TLC of materials with benzene group at higher height than with methyl group ?

The other groups are same in both the material. The solvent system used is also same.

20 December 2023 1,059 1 View

Why is my protein concentration increasing after storage at -20°C?

I have a few proteins that I produce recombinantly in E. coli cells. All of them are disordered proteins and are stored in the presence of 4mM DTT at -20°C before use. One of them also contains...

28 November 2023 2,682 2 View

Why do A431 cells form clumps in propidium iodide staining solution?

I've been trying to study cell cycle using PI (propidium iodide + Triton-X + sodium citrate)b staining solution. I trypsinize my cells (A431: skin carcinoma), fix them using 100% methanol and...

20 November 2023 2,443 3 View

During preparation of emulsion with Ethyleneglycomonostearate (EGMS) and parrafin wax the emulsion is getting paste after certain concentration?

During emulsion polymerization of EGMS and paraffin wax the emulsion is getting paste after certain concentration of EGMS. Why ?

30 August 2023 8,477 2 View

Not getting any protein expression. How to tackle the issue?

I am trying to express a novel GlcNAcT enzyme in BL21(DE3). I have successfully cloned it in pET28a vector and confirmed it through sequencing. I tried following previous protocols for GlcNAcT...

19 July 2023 6,064 2 View

Is there any public database that has both chest x-rays and CT Chest available?

The NIH has separate databases for chest x-rays and CT Chest but I am looking for any public one that has both available to compare some variables between both.

12 July 2023 3,530 2 View

How to obtain sensitivity from cst?

I am working on obtaining sensitivity of microstrip patch in cst but I cannot seem to understand how to obtain the plots in the paper ( page 6044/fig 8). I manually derived the formula for...

27 February 2023 5,663 0 View

Can I do pretest and on the basis of pretest scores can I divide the control group and experimental group?

Hello. I am doing a study to understand the effects of an e-learning module on the academic achievement of students. I am doing a quasi experimental design. I want to know if it is okay to use...

08 February 2023 3,950 15 View

How to conduct qualitative detection of sialic acid using resorcinol method on TLC plates made with silica gel??

I am qualitatively trying to determine the sialic acid using resorcinol on TLC plates. Can someone help me with the process?

26 January 2023 7,825 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Maria Ercibengoa Arana

Buy Mutaflor is a probiotic that can buy by amazon its only component is E.Coli Nissle, culture the powder of the capsules and you will have the colony

Goodluck

It cab be bought by amazon

Jayashree Niharika

It's not available on Amazon in India. Thanks for the suggestion anyway.