Jayashree Niharika

3 Questions 4 Answers 0 Followers

Questions related from Jayashree Niharika

Not getting any protein expression. How to tackle the issue?

I am trying to express a novel GlcNAcT enzyme in BL21(DE3). I have successfully cloned it in pET28a vector and confirmed it through sequencing. I tried following previous protocols for GlcNAcT...

20 July 2023 6,064 2 View

Why i am getting no colonies after transformation in site directed mutagenesis?

I am using Q5® Site-Directed Mutagenesis Kit by NEB. after performing KLD reaction for 5 minutes at room temperature I went for transformation using the high-efficiency competent cells provided in...

29 April 2021 438 10 View

Help needed. E.coli Nissle strain wanted.

I am working on the cell factory approach for the production of certain oligosaccharides. I am looking for Escherichia coli Nissle 1917 (abbreviated as EcN) to clone some genes for this work. I...

01 January 1970 5,902 2 View