Jayashree Niharika

2 Questions 3 Answers 0 Followers

Questions related from Jayashree Niharika

Not getting any protein expression. How to tackle the issue?

I am trying to express a novel GlcNAcT enzyme in BL21(DE3). I have successfully cloned it in pET28a vector and confirmed it through sequencing. I tried following previous protocols for GlcNAcT...

20 July 2023 6,054 2 View

Why i am getting no colonies after transformation in site directed mutagenesis?

I am using Q5® Site-Directed Mutagenesis Kit by NEB. after performing KLD reaction for 5 minutes at room temperature I went for transformation using the high-efficiency competent cells provided in...

29 April 2021 435 10 View