Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...
23 December 2020 4,709 1 View
I made a Pb sensor using carbon quantum dots (CQDs). I add buffer, CQDs, and a given metal to a quartz cuvette of the recommended type. Before adding a metal, the cuvette will emit a yellow-green...
16 December 2020 9,675 3 View
I am looking for recommendations for good resources or guidelines for developing surveys specifically focused on targeting user's opinions or a needs-analysis in the context of engineering design...
12 December 2020 6,698 6 View
Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...
22 November 2020 5,444 1 View
Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...
20 September 2020 1,855 6 View
Hello all. In lab we grow cells to secret mHTT in media that we then collect and subject to centrigufation at 100k G for 2 hours to concentrate the protein and other secreted components of the...
30 August 2020 1,858 1 View
Hello Everyone, Let me preface this question by saying that this is all being done under a legal THC/Hemp license in the state of Colorado. I was hoping to get some advice on how to improve my...
27 April 2020 6,292 1 View
Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...
31 March 2020 3,917 2 View
Hi Everyone, I was wondering if you guys had any ideas/suggestions that I can work with to try to fix my HPLC. I have attached a picture of one of our Blanks (100% ACN). This shape is unusual,...
16 March 2020 6,454 5 View
This is for research about important issues to repatriation of refugees. Literature (or UNHCR documents) connecting forced migration/repatriation to political/civic engagement would be...
04 March 2020 6,616 3 View
Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...
28 February 2021 1,369 5 View
I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...
26 February 2021 8,642 4 View
Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...
23 February 2021 5,654 2 View
Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...
21 February 2021 2,326 3 View
The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...
15 February 2021 3,095 4 View
I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...
04 February 2021 9,152 4 View
Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...
03 February 2021 2,032 3 View
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...
22 January 2021 8,432 3 View
Our task is to numerically invetigate cellular bilayer membrane. In order to numerically simulate behavior of biological complex membrane, we need to have initially constructed membrane. However,...
13 January 2021 7,809 4 View