Hi Amanda,

I'm not sure what exactly is your request, but you'll find our protocol for plasma / serum separation from whole blood samples in this article (Fig 3B):

Article The Sant Pau Initiative on Neurodegeneration (SPIN) cohort: ...

Kind regards,

Daniel

Hello

I dont understand what you really ask. Protocol for plasma or separation from whole blood? if yes, why you frozen the samples (-20 c)?

With these samples, you only can have blood lysate, not others.

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524540/pdf/nihms-707543.pdf

http://www.cinj.org/sites/cinj/files/documents/C4ProcedureForSerumAndPlasmaSepartion.pdf

http://www.pughlab.org/sites/default/files/PughLab_2016.01.005_Plasma%26BuffyCoat_Separation_IC.pdf

Thanks

Thank you for all the answers!!