I study tissue culture of African Ebony. i do somatic embryogenesis with different explants (Leaves, Cotyledons and buds flowers). I need to complete my study with histology.
You can see it with the naked eye when you see the phases of embryogenesis (Global, Torpedo, heart and cotyledon phases). Thank you and good luck.
Histological observations of embryonic calli show the formation of meristematic centers with anticlinal and periclinal divisions. Unlike the non-embryogenic cells which are large, elongated and vacuolated, embryogenic cells are generally small, isodiametric and densely filled with cytoplasm.
Simultaneous stainingwith Evan's blue and Acetocarmine which stains non embryogenic and embryogenic calli respectively
Embryonic cells are characterized with high content of starch which can be easily observed by binicular. Moreover, thick cell wall in aggregated cells called embryonic cluster are the most potential cells to pass the embryogenesis steps including globular, heats, torpedo and finally embryo.
If, the culture is suspension culture, cells can be easily detected, but if the explant is a specific tissue, you can make transects using microtome, to observe sub-epidermal histological modifications where most embryogenic cells are presented.
In the following link, you can find a chapter of in which very illustrative pictures indicating cell differentiation through somatic embryogenesis can be found.
https://www.sciencedirect.com/science/article/pii/S0928342096800085
@ Tchouga Ornella Alvine
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