Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Reinforcement learning toolbox?

I am looking for Matlab Reinforcement learning Toolbox, Can anybody help me? I will b so thankful.

31 May 2020 3,767 3 View

Which module is better for parallelism numpy functions in Python?

Which you recommend?Numba,Joblib,Dask and etc.Why?

20 March 2020 4,529 4 View

Can anyone advise if there is any company that sells a specific antibody for the HLA DRB1*1501 allele?

Hi everyone, I would appreciate if someone can give me some advise regarding Can anyone advise if there is any company with a specific antibody against HLA DRB1*1501 allele (DR 15 allele HLA...

29 November 2019 1,923 2 View

In Rev manager with continuous data, what number do we have to put in the Mean part while we have two pre and posttest means in the main articles?

I am working with Rev manager 5.3, doing a meta-analysis on 8 studies that each compare 2 psychological treatments and found no difference between them.

25 September 2019 6,804 1 View

Would you remind me some universities or professors for study a phd about food science industry for astronauts?

thank you i am narges heidari.

23 November 2018 3,300 3 View

Have complex values of phonon frequencies any meaning?

I have calculated phonon frequencies of 2D sheet by analytical, classical solution as what we learned from basic solid state texts. But I have complex values for frequencies out of Gama point....

20 April 2018 4,131 6 View

In known group comparison/differences technique, what is the sample size for each group?

I need to use the known group differences to check the validity of the constructs as part of developing a new instrument. The instrument has seven first-order constructs and one second-order...

03 March 2018 4,179 2 View

Does Bio-Resonance really work?

Bioresonance is a non-invasive therapy which involves placing electrodes on your skin for diagnosis and treatment. The electrodes are connected to a Bicom machine which checks the energy...

24 February 2018 6,691 47 View

How can I make CD14 free ( getting rid of CD14 binding) after sorting Monocytes with FACS sorter? Any protocol for max yield and highest viability?

27 September 2016 7,644 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View