Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Reinforcement learning toolbox?

I am looking for Matlab Reinforcement learning Toolbox, Can anybody help me? I will b so thankful.

31 May 2020 3,767 3 View

Which module is better for parallelism numpy functions in Python?

Which you recommend?Numba,Joblib,Dask and etc.Why?

20 March 2020 4,529 4 View

Can anyone advise if there is any company that sells a specific antibody for the HLA DRB1*1501 allele?

Hi everyone, I would appreciate if someone can give me some advise regarding Can anyone advise if there is any company with a specific antibody against HLA DRB1*1501 allele (DR 15 allele HLA...

29 November 2019 1,923 2 View

In Rev manager with continuous data, what number do we have to put in the Mean part while we have two pre and posttest means in the main articles?

I am working with Rev manager 5.3, doing a meta-analysis on 8 studies that each compare 2 psychological treatments and found no difference between them.

25 September 2019 6,804 1 View

How to find/calculate the activation energy from this constant (in Arrhenius equation) ?

Guys, I've seen the Modified Arrhenius equation in several forms. For instance, three kinds are attached. Please consider the first attachment. There is an activation energy (Er). In some cases,...

21 September 2019 5,988 25 View

Would you remind me some universities or professors for study a phd about food science industry for astronauts?

thank you i am narges heidari.

23 November 2018 3,300 3 View

Have complex values of phonon frequencies any meaning?

I have calculated phonon frequencies of 2D sheet by analytical, classical solution as what we learned from basic solid state texts. But I have complex values for frequencies out of Gama point....

20 April 2018 4,131 6 View

In known group comparison/differences technique, what is the sample size for each group?

I need to use the known group differences to check the validity of the constructs as part of developing a new instrument. The instrument has seven first-order constructs and one second-order...

03 March 2018 4,179 2 View

Does Bio-Resonance really work?

Bioresonance is a non-invasive therapy which involves placing electrodes on your skin for diagnosis and treatment. The electrodes are connected to a Bicom machine which checks the energy...

24 February 2018 6,691 47 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View