hello how can i quanify the amount of protein I put into the exosomes by electropopration?
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...
18 July 2024 1,390 2 View
Upper Jurassic of Siberia.
26 June 2024 8,177 0 View
I am trying to clen the free antibodies after immunostaining exosome like nanoparticles. I am trying by ultracentrifugation and sucrose cushion. And I do see that my sample is cleaner but I get...
30 April 2024 5,221 0 View
I added a version of my article (as a preprint, though after review and confirmation of acceptance), which cites my previous article. But now, a published version is added separately, and a double...
05 March 2024 4,583 4 View
I see in the instructions and in some other manuals for poly-lysine coating that slides must be cleaned 'before attempting this procedure. Clean with acidic alcohol (i.e., 1% HCl in 70% ethanol)...
14 February 2024 8,147 4 View
We have a surplus of positively charged slides (Superfrost Plus Microscope Slides) since they did not perform well with small zebrafish brain slices, which were washed off from these slides during...
13 February 2024 2,688 4 View
I recently conducted staining on brain sections of adult zebrafish using Nissl stain. The brains underwent pre-fixation in 4% paraformaldehyde, followed by storage in 75% ethanol at -20°C for a...
12 February 2024 4,114 7 View
I'm new to arXiv and need endorsement to submit an article to the q-bio.NC section of arXiv. Could you please help me? Here is the link to endorse my article:...
03 February 2024 5,527 0 View
Hi, Can someone please suggest a reliable article to use to validate a magnetoelectric device simulation in COMSOL? I am talking about something simple, a laminate/ cantilever with 2 layers (one...
14 January 2024 3,632 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,558 0 View
I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...
19 June 2024 4,210 3 View
What parameters are important in this process?
17 June 2024 4,463 0 View
Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...
29 May 2024 6,551 0 View
i use zeta-view to detect exosome particles,
29 April 2024 9,318 2 View
I am currently preparing research methods for my thesis. My sample is an exosome from Adipose. However, I had difficulty identifying what types of proteins were in it. Can anyone help me?...
19 April 2024 4,524 2 View
As a clinical medicine student investigating the role of exosomes in acute kidney injury, my research has progressed to the point where I need to validate the contributions of exosomal membrane...
15 April 2024 8,330 0 View
15 April 2024 2,628 0 View