Why am I getting signal amplification after ultracentrifugation?

01 May 2024 0 5K Report

I am trying to clen the free antibodies after immunostaining exosome like nanoparticles. I am trying by ultracentrifugation and sucrose cushion. And I do see that my sample is cleaner but I get umplification of MFI after testing it on FACS. I see it after purification on Ultra. Do anybody have an idea why?

Badges
Science topic

More Olga teper-shaihow's questions See All

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

What is a functional blocking assay for an antibody fraction?

I will be grateful if you could explain me or give a reference where I can read about: - What steps a functional blocking assay could include? - Are there other possibilities to prove presence...

17 November 2020 3,032 3 View

Should antibodies against the receptor binding domain of a spike protein bind to a complete spike protein?

The receptor binding domain of the coronavirus spike protein has been expressed in Escherichia coli. Antibodies were obtained to the purified protein. These antibodies do not recognize the spike...

13 October 2020 327 5 View

How to perform overlay from different experiments in FACS Diva?

Dear colleagues! I am in desperate need to do overlay data collected on different days in FACS Diva (need to compare some markers expression levels on different days of cell growth). The settings...

28 July 2020 1,779 3 View

How to correctly choose population strata and interpret results for the detection of loci under selection from RADseq data using Bayescan?

I am working on the analysis of fungal populations sampled from 12 geographic locations using RADseq data. The dataset contains 7439 polymorphic loci with ~10 snps per RAD locus (75,482 snps...

19 May 2020 643 3 View

Good entry literature on philosophy and/ or ethics of technology?

Hello, I am searching for a good introduction or overview on the topics, methods and research of philosophy or ethics of technology (separately). Any suggestions? Thanks in advance! Hallo, suche...

23 April 2020 5,096 1 View

What will be the real consequences of the COVID-19 pandemic on corporate credit rating?

We know that with Basel all banks adopt a rating system to evaluate their loans and to calculate their regulatory capital. companies have always had problems with the rating calculated by banks...

08 April 2020 9,034 7 View

Editing experimentsl and control cells with the lentivirus, I have prevailing signals from cell immune response on virus. How can that be?

Hello everybody! We have modified cells with the lentiviral construction and then analyzed the result by RNAseq. I've made gene set enrichment analysis in R-package fgsea. And obtained the result,...

03 March 2020 9,876 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View