03 March 2018 2 10K Report

Is there anything that i could add to the stripping buffer to make it more efficient?

I use the following protocol as prescribed by thermo

  • Warm the bottle of Restore Western Blot Stripping Buffer to room temperature.
  • Place the blot in Restore Western Blot Stripping Buffer and incubate for 5 to 15 minutes at 37°C. Use a sufficient volume to ensure that the blot is completely wetted (i.e., ~20mL required for an 8 × 10cm blot).
  • Remove the blot from the Restore Western Blot Stripping Buffer and wash in TBS or PBS.
  • After determining that the membrane is properly stripped, the second immunoprobing experiment may be performed.

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