Hello,
Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?
I have a Karl-Fischer reagent (for volumetric titration, which is a one-component reagent) with a titer of 5mg/ml. All references unanimously say the Karl-Fischer solution loses its capability...
02 July 2024 5,044 3 View
Greetings and Regards I have a question about bibliometric articles about public health or nursing. If any of the researchers have experience or knowledge in this matter, please tell me. Thank You
15 June 2024 2,795 1 View
Hi everyone Is there any guideline for method validation for cosmetic? I wanna know about criteria for that.
31 May 2024 1,990 2 View
My vector is 7000 bps, and my insert is 1300 bps
28 May 2024 831 4 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its...
11 May 2024 6,310 1 View
Hi Have a nice day I want to determination PAAs and PAHs in tatoo. Would you present and introduce methods to this?
02 April 2024 6,772 0 View
"Hi, I gasify the polyethylene terephthalate granules and mold them using a hot press at a temperature of 260 degrees Celsius to prepare a bulk sample. However, after molding with the hot press,...
31 March 2024 8,668 0 View
I have a dataset from lung cancer images with 163 samples (2D images). I use the fine-tuning of deep learning algorithms to classify samples, but the validation loss did not decrease. I augmented...
21 March 2024 8,344 6 View
Hello, I want to dissolve polyphthalamide in a solvent. I tested different solvents, formic acid, toluene, DMSO, alcohol-based solvents, etc. under high temperature and time, but it did not...
12 March 2024 3,030 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View