Hello,
Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?
I have functionalized MWCNT via both acidic and noncovalent rout, For acidic treatment I have used H2SO4:HNO3 (3:1) while ultrasonication at 55 C, and in non-covalent I used Triton x100, but the...
27 January 2021 4,497 3 View
I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.
26 January 2021 9,096 5 View
I was wondering if anyone has deployed Athena IE in surrogate modeling or metamodels for LCA. There are many research involving data-based models for predicting energy consumption, but I haven't...
23 January 2021 2,267 1 View
Hi everyone, I wanna know about creating the Graphene model in CST. In: Macros -> Materials -> Create Graphene Material for Optical Applications. What is the exact formula that is used in this...
25 December 2020 1,938 4 View
Hello everybody I am going to extract MCPD in oil ,but I havent pressurized liquid extraction. How can I extract MCPD in oil and determination with GC-MS?
20 December 2020 7,389 2 View
Hello I'm working on SAR Images Co-registration.I want to divide my image into two looks with the spectral diversity method. But I did not find its complete formulas in any article. Does anyone...
13 December 2020 3,270 4 View
I am looking for opportunities to expand my research. Please let me know if you have any information. Just a note, I am available for collaboration on papers and projects.
19 October 2020 5,887 1 View
Dear research community, I am using a data file from DHS survey and I need to adjust for cluster sampling design. I am using SPSS version 27. When I weight the data based on the weights that have...
09 October 2020 1,383 3 View
I have already mentioned that i am one of the author of Mymensing med J 2014 Oct;23(4):770-773. Article titled "Association of infertile patients having polycystic ovarian syndrome with recurrent...
04 October 2020 2,148 1 View
Hi everyone need your help.thank you
22 September 2020 8,704 4 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...
24 February 2021 5,183 1 View
For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?
23 February 2021 3,616 2 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View
I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...
19 February 2021 828 6 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...
14 February 2021 3,129 8 View