Kamran Hosseini

6 Questions 6 Answers 0 Followers

Questions related from Kamran Hosseini

What is the reason for the low number of Hek293T-GFP+ cells after transfection with protamine sulfate?

29 January 2023 2,774 0 View

Comparison between fluorescent microscopy and FACS to evaluate GFP+ expression in calcium phosphate method

Hello Dears Good time I transfected HEK293t cells with pLeGO vector containing GFP to investigate the calcium phosphate method. When examined with a fluorescent microscope, the brightness of my...

01 January 1970 7,153 3 View

Electroporation buffer for SH-SY5Y and U87-MG cell lines

Hello have a good time My goal is to transfect SH-SY5Y and U87-MG cell lines using electroporation method. Has anyone had experience working with these cell lines? And what electroporation...

01 January 1970 167 2 View

The reason for the broadening of the PCR product band on the gel

Hello Good time everyone I ran the cloning product on a 1% gel and observed the following band. Can anyone tell me what is the reason why my band has widened?

01 January 1970 3,026 4 View

Protein expression with deletion of one or two alleles in autosomal recessive diseases

Hello friends Good time In an autosomal recessive (homozygous) disease, is the protein activity completely lost by deleting only one allele of the x gene, or should both alleles of the x gene be...

01 January 1970 1,095 0 View

Detailed molecular mechanism of Cas9 enzyme

Hello everyone Can anyone explain molecularly how the Cas9 enzyme cuts exactly three nucleotides downstream of the PAM site?

01 January 1970 2,767 0 View