Hello Can anyone suggest a good way of inducing oxidative stress in mice to exhibit proteinurea phenotype in the mice?

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Von mises stress not showing for (key in label ) node,after selecting probe values?

The job runs(shows von mises stress 75% to the highest 363.9 Mpa) upto the yield strength (361 Mpa) although it has a ultimate strength (469.29 Mpa)specified. Whenever i try to take some data of...

05 April 2024 6,614 0 View

Trying to draw a pipe bend angle other than 90 degree in abaqus 2017 .How can it be done ?

There is no video on youtube that is helping me regarding this issue.Which of following icon in the attached screenshot can help me with it ?

01 April 2024 9,418 0 View

In abaqus 2017 ,my simulation job runs but it is not showing the 75% average von mises stress for any kind of time frame.Can anyone help with it ?

1.Fixed boundary condition at both end of the pipe elbow. 2.Pressure applied is 30 Mpa. 3.Hex ,sweep mesh applied.

18 March 2024 5,935 2 View

Could anyone recommend materials that have been successfully used to mimic the mechanical properties of bone and neural tissue scaffold?

Hello ResearchGate Community, I am currently working on my final year undergraduate project, which involves the compression testing of tissue scaffolds, specifically focusing on neural and bone...

02 March 2024 4,600 1 View

How to do GCMS of gasoline contaminated soil?

I want to know proper process for the concentration of gasoline present in soil and after removal of gasoline, how much quantity left in the soil. So, can anyone please tell me the detailed...

05 February 2024 9,718 1 View

How to detect high volatile organic compounds? Is there any method except GCMS?

I want to know the composition of high volatile compound present in soil. So, which technique will give me the right information of all organic elements present in that high volatile compound? As,...

30 October 2023 9,035 1 View

Immunohistochemistry - Mouse Brain: Queries?

Currently, We are using free floating section in 12-well plates and small baskets to transfer tissues from one well to another. I have a few specific queries: Could you please provide insights on...

03 August 2023 5,523 2 View

ABC Vector Kit Expiration for Immunohistochemistry (IHC) ?

I am currently working on IHC for Per1 protein expression in the suprachiasmatic nucleus of mouse brain coronal sections 40um thickness. The ABC kit that I have, has the expiry date of June 2022....

29 June 2023 3,270 5 View

For Immunohistochemistry, how do you decide the DAB exposure time?

I am working on Immunohistochemistry for Per1 protein expression on mouse brain coronal sections (40microns thickness, free floating). In the protocol that I'm following, it says that the DAB...

29 June 2023 2,647 4 View

How many whole Mouse Brain Sections (40microns) can be used per well in a 12-well plate for IHC?

I am working on Immunohistochemistry on mouse brain and have sectioned 40 microns thick sections as per the protocol. I will be using 12-well plates. How many sections are advisable to be kept per...

12 June 2023 9,602 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Santhana Krishnan

Anupama Priyadarshini Article Transgenic mice overexpressing glia maturation factor-β, an ...

Article Oxidative stress-induced JNK activation contributes to proin...

Kindly refer this papers. Tq

Islam Md Sadikul

Article Wnt/β-catenin links oxidative stress to podocyte injury and ...

you will get full article in sci-hub

Yeshveer Singh

Hi Anupama

Go through these links, they might help.

https://www.hindawi.com/journals/omcl/2016/7978219/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014303/