RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View

Correlation plot between Gene of Interest and miRNA?

Please suggest portals to retrieve normalized-quantile expressions of micro-RNAs (in RPM).My aim is to construct a correlation plot between the gene of interest and microRNA. I searched in human...

28 October 2020 6,398 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

How can I strip off bound antibody from live cell?

I have labeled MCF-7 cells with a certain cell surface antibody and I want to unconjugate the antibody from the cell surface without affecting the cell viability.

20 August 2019 2,426 2 View

Power as boundary condition?

Hello Everyone, I have a pipe from which some fluid is flowing. At the outside wall of a pipe, I have some electrical devices that are touching the pipe from the outside. The power...

10 April 2019 4,099 3 View

How Carrier and Code NCO generates new frequency and phase?

Hello Everyone, Can someone please explain me how the output coming out of PLL or DLL loop filter is utilised by the NCO to generate new frequency using the look-up table. I have read it in...

17 June 2018 8,847 2 View

What is the relation between lattice parameters and particle size in manganites??

The change in particle size is resulting in change in unit cell lattice paramaters in manganites. Please explain it in terms of crystallography and why is it so.

28 February 2018 1,590 3 View

How can we interpret LC MS data?

I have TIC, EIC and MS data. How to create a library of compound in my plant extract?

21 December 2017 1,418 4 View

What should be the appropriate age and weight of albino rats for inducing Type 2 Diabetes ? Also, which albino rat is more appropriate, Wistar or SD?

This is regarding my research on effectiveness of my treatment on type 2 induced albino rats

22 September 2017 5,424 2 View

How do I prepare mice brain homogenate to run into HPLC

I need in details the chemicals required and the amount

03 August 2017 4,635 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View