I want to prepare aqueous solution of mobile phase of o.o4M Brij-35 and this mobile phase has to be adjusted to pH 7.4 with o.5M phosphate buffer which will be prepared with NaH2PO4 and Na2HPO4 solutions.

so, Can anyone help me to prepare phosphate buffer solution of 0,05M using NaH2PO42H2O and Na2HPO4.7H2O with pH of 7.4 ?

waiting for your favorable response,

thanks

serge