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In my Lab we are constructing an RNAi vector ( pPOTLX-1 ) and every time we test LR recombination results using PCR primers and NPT II primers to test the presence of our inserted gene we get...
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I want to isolate a gene from the potato which has 20 gene that are 90% identical to the one i want to use ,How will i design my primers and make sure that i have only extracted my desired gene...
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