Leonce Dusengemungu

4 Questions 7 Answers 0 Followers

Questions related from Leonce Dusengemungu

How to Identify and verify a successful hairpin constructs ( RNAi vector)?

In my Lab we are constructing an RNAi vector ( pPOTLX-1 ) and every time we test LR recombination results using PCR primers and NPT II primers to test the presence of our inserted gene we get...

07 July 2018 4,981 0 View

Is there a more advanced technique for potato virus elimination through meristem tissue culture?

In our Lab we are trying this meristem tissue culture technique for potato virus elimination, we are using binocular microscope and we have realized it is time consuming and more likely...

03 March 2018 2,534 4 View

Is there some TotalRNA in these pictures below?

I'm using RNAprep Pure Kit for purification of total RNA from plant cells and tissues, My samples are from potato leaves

09 September 2017 9,974 7 View

Is there any way of designing primers for target gene while it has so many other identical gene ?

I want to isolate a gene from the potato which has 20 gene that are 90% identical to the one i want to use ,How will i design my primers and make sure that i have only extracted my desired gene...

08 August 2017 2,878 7 View