10 Questions 6 Answers 0 Followers
Questions related from Aakif Inayat
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
08 July 2024 2,909 1 View
Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...
16 June 2024 1,462 3 View
i need some help setting up my culture plate having negative controls,I am going to infect HEK293T cells in a 6-well plate... to one well I will add my lentivirus particles(packaged already), and...
16 April 2024 7,183 5 View
Hello dear researchers,i have transduced my HEK293T cells with IL-2 and then puromycin screening was done, the cells were showing good egfp flourescence ,but then due to some reasons I have to...
15 April 2024 7,252 2 View
Hello to all of the researchers, i need to isolate and grow T lymphocytes from swine lymph nodes. I need you people to guide/help me or send me any protocol/video for T cells isolation from wine...
25 January 2024 1,346 1 View
Hello dear researchers, i am generating a stable cell line of HEK293T cells using lentivirus, i want to ask about the puromycin kill curve,if i seed my cells in a 12-well plate on day 1, Q1) when...
05 January 2024 3,070 4 View
Hello, i have grown my HEK293T cells in 4 wells of a 24-well plate and added different serial dilutions of lentivirus(containing my gene and GFP), now i want to perform FACS analysis so for this...
01 December 2023 8,827 2 View
Hello to all researchers, i need to do lentivirus titration and for this I need 10 fold serial dilution i.e 10^-1 to 10^-4...and i have my lentivirus stock stored with me...second thing is if i...
29 November 2023 9,848 1 View
i have my gene of interest in this way(picture in attached files), Now I am confuse about whether i can use this whole thing to construct my one the 3 lentivirus packaging vector named pLenti...
04 September 2023 7,877 3 View
hello i am a new master's student in an immunology lab and I have been assigned a topic to establish a stable cell line for swine interleukin 2 gene expression, but I do not know how to start my...
16 August 2023 7,525 2 View