Hi,
I need an immortalised human cell line that can be differentiated into sensory neurons. I found that the (only?) one available is the HD10.6. Do you know where I can find/buy it?
thank you in advance for your help
As your question made me aware of the existence of this cell line I created a Cellosaurus entry for it. Here is the preliminary version and below a tentative answer to your question.
ID HD10.6
AC CVCL_WG82
RX CelloPub=CLPUB00486;
RX PubMed=10377351;
RX PubMed=28404842;
CC Characteristics: Immortalized dorsal root ganglion (DRG) cell line that in the presence of doxycycline can differentiate into sensory neurons with nociceptive features (PubMed=10377351).
CC Characteristics: Susceptible to infection by herpes simplex virus 1 (HSV-1). A quiescent state resembling latency can be established in the HSV-1 infection in the presence of acyclovir (PubMed=28404842).
CC Doubling time: 1.2 days (PubMed=10377351).
CC Transfected with: UniProtKB; P01110; Avian myelocytomatosis virus MC29 v-Myc.
CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC Transformant: v-Myc; tetracycline-regulated.
OX NCBI_TaxID=9606; ! Homo sapiens
SX Male
AG
thank your for your reply! I will try to contact Celgene asking if it is possible to have them!
Dear Camilla Boschian did you get the cell line from Celgene, please reply.
Its a shame :-(
Unfortunatly my experience is that this often happens when you try to contact a company to get information or a reagent: you generally do not get any answer the first time. But if you insist and send emails to different adresses you generally do get a reply. So try again!
Camilla Boschian, thank you for the reply
Amos Bairoch, Sure.. will try again
Hi, I was wondering if someone had success in the meanwhile with getting a response from cel gene or maybe even got the cell line?
Thank you!
Caroline Perner Unfortunately they never replied to my email and obviously I did not get the cells!
Hi all, not sure if anyone succeeded in finding these cells but in a recent review of sensory DRG lines by Haberberger et al. Article Immortalized Dorsal Root Ganglion Neuron Cell Lines
they are described as not being available to the general research community.This tallies up with CelGene's lack of response, it's immensely frustrating as there must be some in a freezer somewhere! They were used as recently as 2020 see article : https://europepmc.org/article/med/31694955
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