Kathi Ging

5 Questions 6 Answers 0 Followers

Questions related from Kathi Ging

Is polybrene for lentiviral transduction toxic to human fibroblasts? If you use it, what working concentration do you suggest (5-8 ug/mL)?

I would like to enhance transduction efficiency in a human fibroblast line. Do you have experience with using polybrene or would you suggest protamine sulphate? What is the usual working...

31 May 2023 5,418 5 View

Is there a way to inactivate/quench TagBFP?

We want to perform an enzymatic activity assay using the fluorogenic substrate 4-MU-beta-glucopyranoside (end-point readout, measurement of fluorescence at 440nm). The cells we are using for the...

22 November 2020 3,834 0 View

Have you ever experienced problems in dissolving resorufin beta-glucopyranoside?

We want to set up an activity assay for beta-glucocerebrosidase. We are experiencing difficulties in fully dissolving resorufin beta-glucopyranoside. We tried it in acetate buffer, methanol and...

30 May 2020 8,522 0 View

What are the advantages and disadvantages of RealTime-Glo vs CellTiter-Glo Cell Viability Assay? Which reagent is more suitable for which application?

We would like to assess cell viability and denisity in a 384 well-plate format.

27 January 2020 4,131 2 View

What is the optimal concentration and incubation of MG132 in HEK293 cells?

I would like to test whether the stability of my protein of interest is increased upon inhibition of proteasomal degradation. I transiently transfect HEK293 cells with my protein of interest (a...

11 April 2019 3,855 5 View