Phylogenetic analysis of the gain/loss of genes along different species lineages?

To identify the gain/loss of genes in a set of species? For example, I am interested in identifying the list of tRNA genes which are gained or lost among different strains of bacterial species or...

05 June 2013 5,629 4 View

How do I find shine dalgarno sequences for prokaryotes?

Is there any tool to identify it or Is there any database with relevant information on it?

02 March 2013 3,099 30 View

I am interested in collecting the physiological pH values for Prokaryotes (e.g. bacteria).

For example, some bacteria that thrive in extreme salt water environment will have different pH from other bacterial species in other environments. Is there any database available with these values?

01 February 2013 3,343 0 View

How to find early and late proteins in any species?

Do early proteins represent highly expressed proteins and late proteins represent lowly expressed proteins? Am I correct? Is there any reliable source? On what basis the proteins are decided that...

31 December 2012 4,125 1 View

How to carry out chi square test for this data

I am trying to find, is there is any statistical difference between these two sample groups of data. For example, GCC(A) codon is highly over used in group1 compared to group2. How to get p...

10 November 2012 4,535 6 View

How to find the over used codon from each codon family (2-fold,3-fold and 4-fold) for 10 different strains? I use SAS tool.

10 November 2012 6,333 0 View

What software can I use to do statistical analysis for correspondence analysis?

Correspondence analysis (Multi variate analysis) Currently I am planning to learn correspondence analysis (CA) for my research work. Can anybody teach me how to perform CA and mainly how to...

07 August 2012 1,134 12 View

Temperature data for all bacteria?

Is there any database or research team working on developing a database of all temperature-related data for all types of bacteria?

07 August 2012 6,259 18 View

I want to learn correspondence analysis and multivariate analysis?

If the above concept is explained in biological example, it will be more helpful for me.

05 June 2012 1,270 3 View

Is there any database to get the highly expressed and poorly expressed genes?

For any species?

05 June 2012 9,604 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

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17 February 2021 4,217 3 View