So first I am doing ANOVA to find any differences between the groups (i.e. between 2/3/4-fold codons) and if the difference is significant then using “Tukey’s method” for carrying out multiple comparison tests for example between 4 valine codons and find out which codon ranks first.

I have attached the SAS output for your reference.

The p value in the first part of the output is very less, so there is a significant difference existing between the 4 groups of valine codons. Tukey’s method has grouped the 4 codons based on descending order and has given different letters for 4 codons of valine.

With this output, I figured out that GUU (V) codon is significantly over utilised from other synonymous codons. But I am not sure about significance level value for this over represented codon. At what significance level is this codon over used? Can anyone help me on this query?