Does somebody have experience in inducing the microglial activation by systemic LPS injection in rats? Mice seem to respond well to LPS, but rat do not demonstrate neuroinflammatory changes in response to i.p. LPS injection. The data in PubMed concerning this issue are very sparse, but there is still a possibility that some negative results remained unpublished.
V. A. Aniol
Elena, do you think that blood-brain barrier can't attenuate the LPS impact on microglial cells in vivo?
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Agnes Petit
Hello there
I personally studied this topic. By injecting LPS 2 mg / kg ip to C57BL6 micee, you obtain a clear neuroinflammation 24-28hrs after injection. Microglia are activated, proinflammatory cytokines increased, in different brain regions.
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