Is it possible that rats and mice differ in their susceptibility to LPS (E.coli) effects? We have mentioned serious microglial activation in the brain (increase in the number and cell area of microglial cells) after intraperitoneal LPS injection in mice (2 mg/kg), but rats (Wistar) seem insensitive to LPS under the same conditions (I mean, in the CNS - no activation of microglia measured by cell area and cell number). It was rather surprising to us since we did not suspect such an inter-species difference in the course of inflammatory response. Do you have any assumptions about why it could happen, given that all experimental conditions were similar for rats and mice? Has anyone noted the difference in the timecourse and/or intensity of inflammatory response between these species? I would be grateful for any ideas.
It obviosly can. Susceptibility to LPS varies depending on genetics of the host. Even different strains of mice respond differently to LPS and to different types of LPS.
State of neuroinflammation depends on LPS (from which bacterial strain it is produced) as well as on different strains of mice or rats. Moreover, it depends on time of assessment. There are several articles written by Prof. G. A. Rosenberg.
I think this will help you.
I think that this will basically depend on the pattern of expression of TLR4 in each animal and the cascade initiated after it. So in theory, it can be very different.
Well, many thanks to all who shared their ideas. Now I realize the exciting diversity of biological responses in animals.
Actually, I was mainly interested if somebody had an experience in inducing the microglial activation by systemic LPS injection in rat? The data in PubMed concerning this issue are very sparse, but there is still a possibility that some negative results remained unpublished.
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