Has somebody a home-made protocol for mycoplasma testing by PCR?

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How are DNA Ladders predetermined?

How do companies or scientists predetermine DNA ladders for Gel Electrophoresis? It makes no sense how they would know? I mean DNA is so incredibly small. As it separates in the gel how can they...

30 May 2024 1,317 0 View

Using BL21(DE3)PlysS without adding glucose?

Dear all, I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?

05 May 2024 3,465 0 View

SAP2000/ETABS : Why is my truss taking excessive values of axial force?

I modelled a 3d truss with one storey concrete structure attached to it as shown on the model. I know that the one storey concrete structure changes the rigidity and the flow of the forces on the...

02 January 2024 6,810 1 View

How to work out initial effect size?

Hi I am trying to work out what my effect size was for my study I have the sample size, power etc... its there a program that can work out what it is?

04 December 2023 9,249 3 View

Plasmid purification -too much isopranolol in endotoxin wash buffer?

We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?

04 December 2023 9,317 1 View

The effects of nitrogen in pig manure on soil?

How does the nitrogen in pig manure effect the soil

27 November 2023 1,084 8 View

What does C. diff. RT 014/020 in research papers refers to?

Hi everyone, In my readings about C. diff. ribotypes, I found a ribotype the researcher refers to it as RT 014/020. I wonder if it refer to 014 and 020 strains or 014 to 020 strains or what...

12 November 2023 3,253 0 View

What could be a small molecular weight sized band on SDS-PAGE?

Hello everybody, I purified my protein and when I run it on SDS-PAGE, a small molecular weight sized band (10 kDa) appears. Do you have any idea what that could be? an image of the SDS-PAGE is...

09 July 2023 5,325 3 View

Do I need to comply with the provision for Seismically Compact Section for Trussed Tower?

Hi! I am checking the structural integrity of an existing trussed tower ( 17m high ) using SAP2000. I found out that the main angle bars used are not within the provision for seismically compact...

04 July 2023 9,566 1 View

Why I don't get mutant colonies following quick change site-directed mutagenesis?

I have been working on site-directed mutagenesis for a few months now, without success. I used a High-Fidelity DNA polymerase to amplify my vector with an insert and saw the target band after the...

13 June 2023 8,458 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View