I'm trying to use Mannitol as a positive control for blood brain barrier disruption in a piglet model. I've seen a lot of work done in rat but that involved cervical artery catheterisation and large volumes of drug. I would prefer to do IV or subcut but am not sure on the dose required.
Any experiences working with mannitol would be greatly appreciated.
Hi Kate,
I.V. Mannitol was and is still used to temporary "prevent" BBB disruption in large (or so called malignant) hemisphereic stroke or in other neurocritical diseases with BBB break-down and brain swelling (long-term outcome changes were disappointing). It works only temporary to decrease clinical makers like ICP most likely by its onkotic pressure (so in Neuro-ICUs it is sometimes life-saving). Therefore, I.V. injections may be critical for your purposes to disrupt the BBB. However, Paracelsus is calling: It all about the dose.
I.P. can not work at all, because mannitol as a large molecule should not enter the circulation that good. It is rather made for not crossing endothelial barriers.
To disrupt BBB with mannitol you may need rapid changes of mannitol and thereby the onkotic pressure in the brain cirulation, then mannitol have a paradox effect leading to an "opening" of BBB. That means I.A. mannitol injection or as a compromise I.V. injection is the only way to bring it to the brain circulation in a rapid and higher dose.
I hope these information will help a bit for planing your experiments.
Best Bert
_______________________________________________
Bert Bosche, MD/PhD
Specialist in Neurology, Neurocritical Care,
Neurosurgical Research Fellow - Post-doc Scientist
in the Laboratory of Prof. Dr. R. Loch Macdonald
Division of Neurosurgery
St. Michael's Hospital and Li Ka Shing Knowledge Institute
209 Victoria St., Room 517
Toronto, Ontario, Canada M5C 1N8
E-mail: [email protected]
Phone: +1 416-864-6060 ext. 77641
Mobil: +1 647-204-4757 or +49 173-7076734
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models